DanioReadout was beforehand half of the Faculty of Science and Technology, however is now primarily based on the Department of Immunology, Genetics and Pathology. The research infrastructure works in shut collaboration with the zebrafish facility at Uppsala Biomedical Centre (BMC), which was inaugurated in 2023 and is at present the biggest facility of its type in Sweden. The actions are primarily targeted on Uppsala University, however DanioReadout additionally engages in nationwide collaborations.
“Our mission is to support research at Uppsala University. Anyone interested in using zebrafish in their project can turn to us, regardless of their previous experience with the model,” says Beata Filipek Gorniok, Operational Director at DanioReadout.
300 particular traces
A central part of DanioReadout’s actions is the genetic modification of zebrafish. Since 2015, the infrastructure has generated almost 300 zebrafish traces – genetically outlined strains with particular, heritable traits used as research fashions.
“This is our most established service. We have extensive experience in generating new zebrafish lines that can then be used in a wide range of research contexts,” says Beata.
The traces are used to check gene perform, illness mechanisms and organic processes, in addition to in additional utilized areas corresponding to drug growth and toxicity testing.
“It is often a close collaboration. We contribute zebrafish expertise and technology, while the research group contributes its disease or cell model,” says Beata.
High-throughput imaging and built-in evaluation
DanioReadout gives superior high-throughput imaging, particularly tailor-made to zebrafish embryos and larvae. The infrastructure has entry to a number of automated imaging methods in addition to gives experience in bioinformatics and picture evaluation.
“We aim to support researchers at all stages of their work, from project consultancy to providing already analysed data. This increases the chances of successful project outcomes,” says Beata.
This permits extra detailed research in areas corresponding to toxicology and drug screening, the place results might be analysed on the organ and mobile stage.