Qilin Fan,1,* Fumei Shang,1,* Chen Chen,1 Hongxia Zhou,1 Jun Fan,2 Ming Yang,2 Xiu Nie,2 Li Liu,3 Kailin Cai,4 Hongli Liu1

1Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China; 2Department of Pathology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China; 3Department of Epidemiology and Biostatistics, The Ministry of Education Key Laboratory of Environment and Health, School of Public Health, Huazhong University of Science and Technology, Wuhan, People’s Republic of China; 4Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Hongli Liu
Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China
Tel +86-27-85871962
Fax +86-27-65650733
Email [email protected]
Kailin Cai
Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People’s Republic of China
Tel +86-27-85871962
Fax +86-27-65650733
Email [email protected]

Background: Intestinal microbiota play a vital position within the growth of colorectal most cancers. However, little is thought in regards to the construction and traits of intestine microbial in colorectal most cancers, particularly in regionally superior rectal most cancers after neoadjuvant chemoradiation remedy.
Methods: Here, we carried out this examine to judge microbial traits between pathologic full response (pCR) (n=12) and non-pathological full response (Non-pCR) (n=45) tumor tissues from sufferers with regionally superior rectal most cancers after neoadjuvant chemoradiation remedy. In this examine, 16S rRNA gene sequencing was used to detect the microbial variety together with Alpha variety and Beta variety. Moreover, we used PICRUSt from the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to foretell the microbial metabolism capabilities.
Results: There was important statistical distinction in PFS between pCR and Non-pCR group (p < 0.05). However, there was no important distinction in OS between pCR and Non-pCR group. The microbial compositions within the each teams have been Proteobacteria, Actinobacteria, Firmicutes and Thermi and Bacteroidetes on the phylum stage. The 5 most predominant genera in each pCR and Non-pCR tissue teams have been Sphingobium, Acinetobacter, Cupriavidus, Thermi and Sphingomonas on the genus stage. The key taxa recognized within the pCR and Non-pCR tissues have been Thermi and Sphingomonadaceae respectively. In addition, a sequence of human disease-related genes have been additionally considerably completely different between pCR and Non-pCR group.
Conclusion: In abstract, we demonstrated the attribute variations in microbial communities between pCR tissues and Non-pCR tumor tissues from regionally superior rectal most cancers sufferers after neoadjuvant chemoradiation remedy. Our outcomes current new alterations within the microbiome in regionally superior rectal most cancers after neoadjuvant chemoradiation remedy, suggesting that it’ll present a brand new perspective for the exact remedy of neoadjuvant rectal most cancers by concentrating on particular microbial species sooner or later.

Keywords: intestinal microbial, 16S rRNA gene sequencing, pathologic full response, neoadjuvant chemoradiation remedy, colorectal most cancers


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